Italy beat Australia for the second time in their history thanks to tries by Melbourne-born Monty Ioane and by Louis Lynagh, the son of former Wallaby Michael Lyagh.
There are eight changes to the team that defeated Japan, with Joseph-Aukuso Suaalii, Max Jorgensen and Taniela Tupou among those to return to the team.
The action-packed calendar reflects the evolving requirements of its Member Unions (Argentina, Australia, New Zealand and South Africa) as well as broadcasters and fans.