Test veteran Tatafu Polota-Nau will link with the Qantas Wallabies in Wales ahead of their three Test Spring Tour of Wales, Italy and England this month.
Polota-Nau has been playing for Leicester in the English Premiership after last featuring for the Wallabies against Argentina on the Gold Coast in September.
Jed Holloway and Brandon Paenga-Amosa will now return to Australia following an intensive training camp at Odawara, Japan, where the Wallabies will be based ahead of the Rugby World Cup next year.
Isi Naisarani, who has been training with the squad, will travel to Europe as a Development Player alongside fellow rookie Rob Valetini.
Naisarani will become eligible for Australia in April next year, and has already been touted as a future Wallaby backrower after several bruising displays in Vodafone Super Rugby and the National Rugby Championship.
In the squad is 116-Test veteran Adam Ashley-Cooper who was named in the Wallabies Spring Tour train-on squad this week after a two-year absence as he bids for a fourth Rugby World Cup bid.
Ashley-Cooper has been playing his rugby in Japan this season, last playing for the Wallabies in the 2016 Bledisloe Cup Test in Wellington.
Brumbies backrower Isi Naisarani will travel with Wallabies as a development player
Wales v Qantas Wallabies at Principality Stadium, Cardiff, Sunday 11 November at 4.05am AEDT
Italy v Qantas Wallabies at Stadio Euganeo, Padua, Sunday 18 November at 12.45am AEDT
England v Qantas Wallabies at Twickenham Stadium, London, Sunday 25 November at 1.45am AEDT
Jermaine Ainsley (1 Test, Melbourne Rebels, 23)
Allan Alaalatoa (30 Tests, Brumbies, 24)
Rory Arnold (19 Tests , Brumbies, 28)
Adam Coleman (28 Tests, Melbourne Rebels, 26)
Jack Dempsey (7 Tests, NSW Waratahs, 24)
Folau Fainga’a (6 Tests, Brumbies, 23)
Ned Hanigan (18 Tests, NSW Waratahs, 23)
Michael Hooper (c) (88 Tests, NSW Waratahs, 27)
Sekope Kepu (100 Tests, NSW Waratahs, 32)
Tolu Latu (10 Tests, NSW Waratahs, 25)
Tatafu Polota-Nau (86 Tests, Leicester, 33)
David Pocock (75 Tests, Brumbies, 30)
Izack Rodda (14 Tests, Queensland Reds, 22)
Rob Simmons (91 Tests, NSW Waratahs, 29)
Pete Samu (7 Tests, Brumbies, 26)
Scott Sio (52 Tests, Brumbies, 27)
Taniela Tupou (9 Tests, Queensland Reds, 22)
Adam Ashley-Cooper (116, Kobe Steelers, 34)
Tom Banks (3 Tests, Brumbies, 24)
Kurtley Beale (81 Tests, NSW Waratahs, 29)
Israel Folau (70 Tests, NSW Waratahs, 29)
Bernard Foley (65 Tests, NSW Waratahs, 29)
Will Genia (97 Tests, Melbourne Rebels, 30)
Jake Gordon (uncapped, NSW Waratahs, 25)
Dane Haylett-Petty (28 Tests, Melbourne Rebels, 29)
Samu Kerevi (22 Tests, Queensland Reds, 25)
Marika Koroibete (18 Tests, Melbourne Rebels, 26)
Jack Maddocks (5 Tests, Melbourne Rebels, 21)
Sefa Naivalu (8 Tests, Melbourne Rebels, 26)
Jordan Petaia (uncapped, Queensladn Reds, 18)
Nick Phipps (70 Tests, NSW Waratahs, 29)
Matt Toomua (39, Leicester/Melbourne Rebels, 28)